Agron 600A - Plant Breeding Seminars
| Date/Time: | Thursday, 28 Apr 2011 from 4:10 pm to 5:10 pm |
|---|---|
| Location: | 2026 Agronomy Hall |
| Cost: | Free |
| URL: | http://www.agron.iastate.edu |
| Contact: | |
| Phone: | 515-294-1360 |
| Channel: | Agronomy Department |
| Categories: | Lectures |
| Actions: | Download iCal/vCal | Email Reminder |
Quality of silage corn is a trait of major interest for corn breeding in Northern Europe. In order to increase breeding efficiency, it is important to locate precisely the loci (QTL) involved in the variation of this trait and to identify, within available breeding material, those that carry favorable alleles at these QTL. The goal of this PhD was to carry out two complementary approaches. First, a meta-analysis was performed on the QTL detected in previous studies issued from publications and Syngenta, in order to refine the definition of chromosomal regions involved in traits related to digestibility. Maize physical map and sequence data were used to identify and position candidate genes for silage quality, and to test their colocalization with metaQTL. A total of 37 and 54 metaQTL were found for digestibility and cell wall respectively and the candidate genes colocalized significantly with the metaQTL confidence intervals. Secondly, association mapping was performed on the regions identified through metaanalysis. The panel used for the association tests was composed of 314 inbred lines representing dent European and American germplasm, including public inbred lines and elite material of Syngenta. More than 100 amplicons were analyzed in the regions of interest over the whole panel and tested for their association with digestibility traits. Association between flowering time and sequences previously studied by Ducrocq et al. (2008) at the Vgt1 QTL region were also investigated to evaluate the properties of this panel relative to the ones previously published and compare different association mapping software. Our results confirm the effect of the Vgt1 region. Nine significant associations were found for digestibility related traits.