Webinar: Nanovaccine Institute series

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Date/Time:Thursday, 02 Dec 2021 from 3:00 pm to 4:00 pm
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"Single molecule interaction forces and reaction kinetics of ligand-receptor systems using AFM on live cells and fast data analytics," Anwesha Sarkar, ISU electrical and computer engineering.

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About the Seminar
Measurement of interaction forces and binding kinetics of ligand-receptor interactions on live cells at single molecular level is extremely crucial for gaining novel biological insights, evaluating potential therapeutic agents for cancer and other diseases as well as for developing new gene and drug delivery approaches. In this talk, I will discuss developing a biophysical method to isolate and measure specific interactions between Discoidin domain receptors (DDRs) which are overexpressed in cancer, fibrosis and other diseases and collagen (ligands of DDRs) in live cells at the single molecule level using atomic force microscopy (AFM). Another commonly over-expressed receptor in diseases which can be targeted in drug delivery by ligand-conjugated nanoparticles is the folate receptor alpha (FR?). In this regard, I will talk about measuring the binding dynamics of this receptor with self-assembled nanoparticles decorated with a folic acid (FA) ligand using AFM and proving that multivalent micelleplexes bind to FR? with a higher binding probability and binding force than monovalent FA. I will also talk about how I have been exploring machine learning approaches to make AFM high throughput and AFM data analytics faster.